pde3b cell extract from sf9 cells overexpressing human full length pde3b  (SB Drug Discovery)

Journal: Iranian Journal of Biotechnology

Article Title: Expression of Recombinant Phosphodiesterases 3A and 3B Using Baculovirus Expression System

doi: 10.15171/ijb.1400

Figure Lengend Snippet: Primers for RT-PCR

Article Snippet: SF9 insect cells were obtained from the School of Life Science, Jiangsu University; SF900-II (Invitrogen, CA, USA); baculo-EZTM Expression Ready Full Length Collection- human PDE3A and PDE3B were purchased from (Orbigen, USA), total RNA Isolation Kit and SuperScriptTM One-Step RT-PCR Systems were purchased from (Invitrogen, CA, USA), primary antibodies for PDE3 and HRP-conjugated IgG (Santa Cruz, CA, USA), Milrinone was obtained from Huzhou Hengyuan Biological limited company, Taq DNA polymerase was purchased from (TaKaRa, China), 125 I RIA cGMP, and 125 I RIA cAMP Kits were obtained from (Shanghai University of Traditional Chinese Medicine).

Techniques: